Journal: PLoS ONE

Article Title: Mechanisms of Nifedipine-Downregulated CD40L/sCD40L Signaling in Collagen Stimulated Human Platelets

doi: 10.1371/journal.pone.0127054

Figure Lengend Snippet: Platelets were pretreated with 5 μ M nifedipine or nifedipine combined with various drugs for 3 min followed by addition of collagen for 6 min. The expression of phospho-P38MAPK and total-P38MAPK (A), phospho-ERK1/2 and total ERK1/2 (B), phospho-HSP27 and total-HSP27 (C) were determined. Date were expressed as means ± SEM ( n = 5). *** p < 0.001 compared with collagen alone group; ++ p < 0.01, +++ p < 0.001 compared with respective collagen+nifedipine-treated group.

Article Snippet: The phospho-HSP27, total-HSP27, phospho-p38 mitogen-activated protein kinase (p38MAPK), total-p38MAPK, phospho-ERK1/2, total-ERK1/2 antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA).

Techniques: Expressing

Journal: PLoS ONE

Article Title: Mechanisms of Nifedipine-Downregulated CD40L/sCD40L Signaling in Collagen Stimulated Human Platelets

doi: 10.1371/journal.pone.0127054

Figure Lengend Snippet: Nifedipine initially activates PPAR-β/-γ followed by increased formation of NO/cyclic GMP and down-regulation of p38MAPK/ERK1/2/HSP27 signaling as well as ROS generation, which then attenuates MMP-2 activity and ultimately inhibits sCD40L release from activated platelets.

Article Snippet: The phospho-HSP27, total-HSP27, phospho-p38 mitogen-activated protein kinase (p38MAPK), total-p38MAPK, phospho-ERK1/2, total-ERK1/2 antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA).

Techniques: Activity Assay

Journal: Nature

Article Title: Neutrophil ageing is regulated by the microbiome

doi: 10.1038/nature15367

Figure Lengend Snippet: Pathways selected for the analysis of neutrophil functions.

Article Snippet: MAPK pathway , BIOCARTA_MAPK_PATHWAY.

Techniques: Coagulation, Activation Assay, Ubiquitin Proteomics

Journal: Nature

Article Title: Neutrophil ageing is regulated by the microbiome

doi: 10.1038/nature15367

Figure Lengend Snippet: Gene set enrichment analysis of selected pathways in aged and activated neutrophils.

Article Snippet: MAPK pathway , BIOCARTA_MAPK_PATHWAY.

Techniques: Coagulation, Activation Assay, Ubiquitin Proteomics

Journal: Molecular Oncology

Article Title: KLK6‐regulated miRNA networks activate oncogenic pathways in breast cancer subtypes

doi: 10.1016/j.molonc.2016.03.008

Figure Lengend Snippet: The effect of KLK6 transfection on miRNA‐mediated signaling pathways. Panels of MAPK signaling and related factors were assayed for expression changes between KLK6 overexpressing and mock cells. In general, expression of mRNAs encoding for transcription ...

Article Snippet: The cDNA was synthesized as previously and was added to the MAPK Signaling Pathway RT 2 ProfilerTM PCR array (Qiagen/SuperArray Biosciences) as per the manufacturer's protocol.

Techniques: Transfection, Protein-Protein interactions, Expressing